Addition of 3' A overhangs to PCR products

If PCR is performed using a proofreading DNA polymerase, such as Pfu DNA polymerase, the product will have blunt ends. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.

Details: http://openwetware.org/wiki/Addition_of_3'_A_overhangs_to_PCR_products